Guidelines on Sterilization and Disinfection Methods by World Health Organization

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N Engl J Med 354:1913–1921, 2006. Sawada A, Takihara Y, Kim JY, Matsuda-Hashii Y, Tokimasa S, Fujisaki H, Kubota K, Endo H, Onodera T, Ohta H, Ozono K, Hara J. A congenital mutation of the novel gene LRRC8 causes agammaglobulinemia in humans. J Clin Invest 112:1707–1713, 2003. Scriver CR, Beaudet AL, Sly WS, Valle D, Stanbury JB, Wyngaarden JB, Fredrickson DS. The Metabolic and Molecular Basis of Inherited Disease, 8th ed. New York: McGraw-Hill, 2001. Smith CIE, Vihinen M. Immunodeficiency mutation databases—a new research tool.

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The value of θmax is, therefore, a measure of genetic distance between two loci. The second parameter, zmax, is a measure of how good that estimate of θmax actually is. 5, linkage is strongly suggested. If zmax is larger than 3, the likelihood that the loci are linked is a thousand times (103) greater than the likelihood that the linkage data are purely the result of chance. Thus LOD scores with zmax of 3 or greater are taken as nearly definitive proof that two loci are linked. LOD score analysis will demonstrate which polymorphic loci of known location are linked to the disease by finding the smallest value of θmax with the largest zmax, preferably 3 or greater.

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