Emerging Infectious Diseases - Vol. 14, No. 3, March 2008 by Centers for Disease Control and Prevention

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Wells of a high protein-binding microplate (Nunc Maxisorp, Fisher Scientific) were coated with 50 μL of infected or uninfected cell lysates at 5 μg/mL in PBS and were left to adsorb overnight at 4°C. 05% Tween by using an automated EL 404 microplate washer (Biotek Instruments, Winooski, VT, USA). After washing, all wells were blocked with 300 μL of Superblock blocking buffer (Pierce, Rockford, IL, USA) in PBS with sodium azide (PBS/Az), after which the dolphin serum samples were applied (1:500 in 1% bovine serum albumin [BSA] in PBS/Az).

16. 17. 18. References 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 404 Hall CB. Respiratory syncytial virus and parainfluenza virus. N Engl J Med. 2001;344:1917–28. McCandlish IA, Thompson H, Cornwell HJ, Wright NG. A study of dogs with kennel cough. Vet Rec. 1978;102:293–301. Fulton RW, Purdy CW, Confer AW, Saliki JT, Loan RW, Briggs RE, et al. , parainfluenza-3 virus, and bovine respiratory syncytial virus. Can J Vet Res. 2000;64:151–9. Nollens HN, Wellehan JX, Saliki JT, Caseltine SL, Jensen ED, Van Bonn W, et al.

To test this hypothesis, results of environmental sampling would need to be correlated with cases of human disease (14,15). Our study provided an opportunity to study risk factors in a population without referral center bias that can occur in centers specializing in NTM care. The predominance of women among persons with NTM disease is consistent with previous reports (12,16). For pulmonary disease caused by MAC, the greater proportion of affected women appeared to reflect the higher proportion of women in the older age strata of the base population of NYPH.

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