By Daryl S. Henderson
Probably no unmarried experimental organism has contributed as a lot to our figuring out of the constitution, functionality, and mechanism of transmission of eukaryotic chromosomes as has the fruit fly, Drosophila melanogaster. In Drosophila Cytogenetics Protocols, prime drosophilists describe, in step by step aspect, all of the crucial thoughts for learning Drosophila chromosomes and recommend new avenues for medical exploration. normal parts lined contain analyses of nuclei and chromosomes in spermatogenesis and oogenesis, fluorescence-activated phone sorting of ovarian follicle cells, analyses of nuclear department cycles in embryos, and analyses of programmed mobilephone loss of life. moreover, there are traditional light-, electron-, and immunofluorescence-microscopic analyses of polytene chromosomes; cytological analyses of larval brains, imaginal discs, and histoblasts; and nonfluorescent and fluorescent in situ hybridization (FISH) to either polytene and mitotic chromosomes. A state of the art mixed FISH and immunolocalization protocol can be vital for locating how DNA series impacts higher-order chromosome structure and eventually gene expression. every one comfortably reproducible protocol bargains a history advent, gear and reagent lists, and tips about troubleshooting and averting pitfalls.
without difficulty obtainable and hugely sensible, Drosophila Cytogenetics Protocols offers a complete cytogenetics laboratory handbook for Drosophila investigators, one who is appropriate not just for newcomers, but in addition hugely informative for professional investigators.
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Extra resources for Drosophila Cytogenetics Protocols
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