By Joseph A. Vinson
content material: A survey of metabolic adjustments of [delta]¹-tetrahydrocannabinol / Sumner Burstein --
GLC and HPLC analyses of cannabinoids in organic fluids and purposes / E.R. Garrett, A.J. Gouyette, and C.A. Hunt --
Detection and quantitation of tetrahydrocannabinol in physiological fluids / Monroe E. Wall, Dolores R. Brine, Joan T. Bursey, and David Rosenthal --
Quantitation of [delta]⁹-tetrahydrocannabinol and 11-nor-[delta]⁹-tetrahydrocannabinol-9-carboxylic acid in physique fluids via GC/CI-MS / R.L. Foltz, P.A. Clarke, B.J. Hidy, D.C.K. Lin, A.P. Graffeo, and B.A. Petersen --
development of the mass fragmentographic strategy for quantification of tetrahydrocannabinol in human blood plasma / Agneta Ohlsson, Stig Agurell, Jan-Erik Lindgren, and Kurt Leander --
A basic method of the research of cannabinoids from physiological assets / J. Rosenfeld --
Quantitation of [delta]⁹-tetrahydrocannabinol and its metabolites in human urine by way of chance dependent matching GC/MS / Donald E. eco-friendly, Fu-Chuan Chao, Kay O. Loeffler, and Saul L. Kanter --
HPLC research of [delta]⁹-tetrahydrocannabinol and metabolites in organic fluids / Seth R. Abbott, John R. Berg, Kay O. Loeffler, Saul Kanter, Leo E. Hollister, Joan Hawkins Abrams, Hugh L. Baras, and Reese T. Jones --
Radioimmunoassay of cannabinoid compounds / C.E. cook dinner --
Antisera raised opposed to tetrahydrocannabinol within the radioimmunoassay of cannabinoids / J.D. Teale, Jacqueline M. Clough, L.J. King, V. Marks, P.L. Williams, and A.C. Moffat --
HPLC analyses of [delta]⁹-tetrahydrocannabinol and 11-nor-[delta]⁹-tetrahydrocannabinol-9-carboxylic acid in human plasma / Jimmie L. Valentine, Owen H.M. Gan, H.C. Nio, and Everett D. Thompson --
Detection and quantitation of [delta]⁹-tetrahydrocannabinol in plasma by means of dansylation and double labeling / J.M. Scherrmann, R. Bourdon, H. Hoellinger, Nguyen-Hoang Nam, and E. Fournier.
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Extra info for Cannabinoid Analysis in Physiological Fluids
The GLC methodology p r e s e n t e d h e r e i n d i f f e r e d from p r i o r s t u d i e s (17) i n t h a t t h e s h o r t 30 cm column o f 3% OV-225 was s u p p l a n t e d by a l o n g e r OV-17 packed column t o be c o n s i s t e n t w i t h t h e d a t a i n t h e l i t e r a t u r e accumulated f o r t h e r e s o l u t i o n o f t h e c a n n b i n o l s (23-25) . However, t h e e f f i c i e n c i e s o f t h e s e p a r a t i o n o f t h e s e two t y p e s o f columns (OV-17 and OV-225) were compared f o r a m i x t u r of the a c i d degradatio d e t e c t i o n .
Both peaks were p r e s e n t t o g e t h e r ; Eoth were a b s e n t when d e g r a d a t i o n s were e f f e c t e d i n HPLC p u r i f i e d w a t e r and when t h e g l a s s w a r e was p r e v i o u s l y s i l y l a t e d ( R e g i s i l ) . The use o f TLC p u r i f i e d 1_ showed b o t h p r o d u c t s 11 and 12. A l s o , when t h e w a t e r used was p r e - e x t r a c t e c T w i t h chloroform, f o r both untreated o r aqueous-silicone ( S i l i c l a d ) coated glassware, both of these products were p r e s e n t i n h i g h e v i d e n c e .
L i p t o n , Μ. , Timmons, M. , W a l l , M. , B r i n e , D. , and Davis, Κ. , Clin. Pharmacol. Ther. 14, 48 (1973). Saad, Η. Υ . , and H i g u c h i , W. , J. Pharm. Sci. 54, 1205 (1965). M a r t i n , A . , and Cammarata, Α . , 1969, p. 237. , " D i s s o c i a t i o n Constants o f Organic Acids in Aqueous S o l u t i o n " , I n t e r n a t i o n a l Union of Pure and A p p l i e d Chemistry, Butterworth, London, England, 1961. , N i l s s o n , I. , and A g u r e l l , S . , Biochem. Pharmacol.