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2001; Osborn, 1963). , 1995). , 1982). , 1979). , 1988). The lipid A can be extracted from the hydrolytic reaction mixture using the solvent of chloroform and methanol (2:1, v/v). 34 X. Wang et al. 2 Extraction of Lipid A from Bacteria Extracting lipid A from purified LPS is considerably laborious and time-consuming. , 2006a). Two extraction solutions are needed in this extraction method. 8, v/v/v) (Bligh and Dyer, 1959), in which glycerophospholipids and free lipid A are soluble. 8, v/v/v) in which lipid A is soluble in the hydrophobic phase, and proteins and nucleic acids are soluble in the hydrophilic phase.
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